The calculation of the sperm concentration was performed according to the number of counted cells and hemocytometer dimensions. Place a small drop on a pre-warmed slide and gently place a cover slip over the drop. Sperm density, seminal plasma composition and their physiological relationship in the endangered Caspian brown trout Salmo trutta caspius. Access restricted to users. Is there really a decrease in sperm parameters among healthy young men? The absorbance read by a spectrophotometer depends on the characteristics of absorbing materials in a sample such as size, shape, and homogeneity, and can be affected by the techniques used. For each sample, the absorbance was measured three times, and an average of these absorbance values was used in data analysis.
Control points in the preparation of semen doses: sperm concentration (2/2)
Disclosure Statement No competing financial interests exist. The presence of mucous streaks may interfere with the counting procedure and suggests inflammation or abnormal liquefaction. Analysis of the results confirmed that this method was efficient, sample-sparing, precise, and accurate for measurement of sperm concentration. National Institutes of Health, Grant number: For zebrafish, we prepared eight separate pooled samples collected by crushing of dissected testes composed of sperm from one to four males for each, and six pooled samples collected by stripping composed of sperm from three to five males for each.
Remove a small sample 5 to 10 ml and place in a clean glass test tube. The mixture is now transferred onto a haemocytometer chamber for leukocytes and the number of peroxidase-positive cells which stain brown is counted. All the analyzes were performed with the aid of R software and Multicomp package to perform the Dunnett test. To test technical contributions to the accuracy of the measurements, three aliquots of a pooled sample were processed independently and no significant differences were found among the absorbance measurements of these samples. The testicular weight of groups supplemented with ethanolic extract and aqueous fraction soluble in methanol was higher when compared to the control. Be sure you count 5 squares on each side of the hemocytometer and then use the average for your calculations.
The animals received commercial rodent chow and water ad libitum. Rrumbullaku Albania Tutor A. Allow the slide to air dry. Nevertheless the several less comprehensive studies have examined the predictive value of traditional semen characteristics for in vivo fertility and concluded that these parameters are predictive of pregnancy outcome 2. The concentration was expressed in millions of sperm per mL Badkoobeh et al. You can thus multiply the average number of sperm over each central counting area by 10, to obtain the number of sperm per ml of diluted sample.